Table I.

Expression profile of cyclic nucleotide PDE subtypes in monocytic cell lines, PBMC, and primary monocytesa

SubtypePBMCMonocytesU937THP-1Mono Mac1
PDE-1A
PDE-1B+++++
PDE-1C(+)(+)++
PDE-3A
PDE-3B++++
PDE-4A++++
PDE-4B+++++
PDE-4C
PDE-4D+++++
  • a +, Expression of subtype detected; −, expression of subtype not detected; (+), weak expression of subtype detected. RT-PCR was used for the detection of mRNA for the subtypes of PDE-1, PDE-3, and PDE-4. Human prostate cDNA, which expresses all subtypes of PDE-1 and -4, was used as a positive control. Human colon cDNA served as a positive control for PDE-3A (19 ). Data are representative of four independent experiments.