Table I.

Phenotype analysis of CD4+ T cell populationsa

PBMCCD4+ T Cells after Negative SelectionFACS-Sorted CD4+ T Cells
CD3+CD4+42.597.8599.92
CD8+14.60.540.03
CD56+9.30.29<0.01
CD20+9.90.080.02
CD14+150.78<0.01
BDCA1+0.50.07<0.01
BDCA2+0.50.14<0.01
  • a CD4+ T cells were purified by negative selection using immunomagnetic beads followed by positive selection of CD4+ cells by FACS. Purity of the cells was analyzed by FACS at each step, using fluorochrome-labeled mAbs directed against membrane markers of putative contaminating cells, as described in Materials and Methods. Results are expressed as a percentage of cell populations and are representative of one of five experiments.