Table II.

CD25 expression in CD4+CD25dim T cells treated with or without PGE2a

Sample No.24 h48 h72 h96 h
CD4CD25dimCD4CD25dim + PGE2CD4CD25dimCD4CD25dim + PGE2CD4CD25dimCD4CD25dim + PGE2CD4CD25dimCD4CD25dim + PGE2
Percentage positive and MFI
I64 (277)40 (71)74 (833)47 (186)91 (1516)59 (559)95 (1091)82 (614)
II59 (125)41 (42)74 (272)39 (64)94 (934)64 (364)96 (1039)74 (473)
III75 (101)55 (36)63 (102)25 (21)71 (719)47 (102)96 (1055)78 (638)
IV69 (144)46 (66)74 (668)50 (282)89 (1599)63 (698)96 (1360)65 (676)
  • a CD25 surface expression and PGE2-mediated modulation of CD25 in CD4+ PBL. Data summarize (n = 4) the analysis of CD25 expression in CD4+CD25dim T reg cells in a time-course flow-cytometry experiment from PBL donors (n = 4), as described in Materials and Methods. Briefly, magnetic bead-purified CD4+CD25dim, pretreated with or without PGE2 were incubated with anti-CD3 plate-bound Ab at the indicated time points. Surface CD25 expression was then measured by flow cytometry. Results are expressed as percentage of CD25 expression within the CD4+ population. Values in parentheses indicate the mean fluorescence intensity (MFI) of CD25 expression based on density plot analysis.