CD25 expression in CD4+CD25dim and CD4+CD25bright following magnetic bead purificationa
CD4+CD25dim | CD4+CD25bright | ||
---|---|---|---|
% Positive | (MFI) | % Positive | (MFI) |
24.2 ± 19 | (51 ± 7.7) | 94 ± 2.7 | (247 ± 113.8) |
a CD25 surface expression and PGE2-mediated modulation of CD25 in CD4+ PBL. Data summarize (n = 8) the CD25 expression of CD4+CD25dim and CD4+CD25bright T cell populations following cell purification (Miltenyi Treg Cell Isolation kit). Results are expressed as percentage of CD25 expression within the CD4+ population. Values in parentheses indicate the mean fluorescence intensity (MFI) of CD25 expression based on density plot analysis.