Table III.

Phenotypic analysis of persistent and nonpersistent T cell clonotypesa

Marker Distinguishing Persistent from Nonpersistent T cellsb
CD28
Percentage of positive T cells (mean ± SEM, %)
Persistent clonotypesNonpersistent clonotypes
65 ± 1034 ± 10
Markers that fail to distinguish persistent from nonpersistent T cellsc
 4-IBBCD30CD70CXCR4
 CCR5CD40LCD71DR3
 CCR6CD45RACD80HVEM
 CCR7CD45ROCD86ICOS
 CD2CD48CD94NKB1
 CD5CD50CD95NKG2D
 CD8ααCD54CD102Notch1
 CD8βCD56CD122PD-1
 CD11aCD57CD127TRAIL
 CD16CD58CD161
 CD25CD62LCD178
 CD27CD69CD231
  • a Phenotypic markers were analyzed by FACS. TCR Vβ Abs or MART-1 tetramer were used to distinguish different T cell clonotypes by direct staining of T cells.

  • b Pairwise analysis of the percentages of persistent and nonpersistent T cells corresponding to dominant clonotypes from seven individual responding patients demonstrated a significant difference in the expression of CD28 using the Wilcoxon signed-ranks test (p < 0.05) without correction for multiple comparisons.

  • c Pairwise analysis of the percentages of persistent and nonpersistent T cells corresponding to dominant clonotypes from seven individual responding patients demonstrated no significant differences in the expression of 45 phenotypic markers using the Wilcoxon signed-ranks test (p > 0.1).