Table I.

Effects of NF-κB and IL-1 inhibition on IL-6 and IL-8 levels in cultured chondrocytes treated with FN-f or IL-1βa

TreatmentIL-6 (ng/ml)IL-8 (ng/ml)
MonolayerAlginateMonolayerAlginate
Control0.4 ± 0.20.5 ± 0.030.3 ± 0.017.5 ± 0.9
FN-f299 ± 1189.4 ± 0.4172 ± 2.191.2 ± 2.3
FN-f+ NF-κBib0.06 ± 2.40.16 ± 0.040.22 ± 0.053.2 ± 0.04
FN-f+ IL-1Ra250 ± 12.281.9 ± 4.6165 ± 5.569.4 ± 8.1
IL-1β380 ± 0.9154 ± 7.5207 ± 0.02146 ± 0.86
IL-1β+ NF-κBi0.26 ± 4.60.64 ± 0.040.21 ± 0.027.7 ± 0.04
IL-1β+ IL-1Ra0.35 ± 4.31.51 ± 0.132.7 ± 0.029.96 ± 0.19
  • a ELISA results shown are mean ± SD for duplicate cultures.

  • b NF-κBi, NF-κB inhibitor.