Table I.

TLR4-dependent HP0175-induced apoptosis in AGS cells

Annexin V-Fluos-Positive Cells (%)
Control6 ± 1a
His-HP017560 ± 5
Irrelevant His-protein7 ± 1
Heat-treated His-HP017510 ± 3
His-HP0175 + anti-TLR4b9 ± 2c
His-HP0175 + i/cb58 ± 7
His-HP0175 + PBd61 ± 6
His-HP0175 + SB203580 (5 μM)7 ± 1c
His-HP0175 + U0126 (25 μM)58 ± 6
HP(WT)75 ± 5
HP(KO)35 ± 2e
Vector control + His-HP017560 ± 2
ASK1 KM + His-HP017520 ± 4f
p38 agf + His-HP017521 ± 5f
Caspase 9s + His-HP017522 ± 4f
IETD + His-HP01756 ± 1c
DEVD + His-HP017510 ± 2c
LEHD + His-HP01759 ± 3c
  • a Results represent the means ± SD.

  • b Cells were pretreated with anti-TLR4 mAb or isotype-matched Ab (i/c) before challenge with His-HP0175.

  • c p < 0.001 vs His-HP0175.

  • d His-HP0175 was incubated with polymyxin B resin as described in Fig. 1 before incubation with AGS cells.

  • e p < 0.05 vs WT.

  • f p < 0.05 vs vector control + His-HP0175.