Table II.

Phenotypic analysis of expanded-Vδ2 T lymphocytes from MRCC patients and donorsa

PatientCD8CD56HLA-DRCD16CD158aCD158bCD158eNKG2DNKG2A
320 (324)41 (254)55.4 (67)11.7 (124)0.7 (23)25.3 (734)1.8 (298)93.4 (125)100 (351)
631.4 (378)25.7 (187)23.1 (98)17.3 (56)2.1 (78)7 (59)3.1 (1102)86.8 (256)41.5 (173)
734.2 (669)14.3 (117)18.5 (522)15.3 (103)9.2 (385)25.5 (632)6.1 (1440)24.2 (81)19 (158)
826.1 (369)99.7 (183)53.5 (59)8.5 (104)1.7 (55)5 (101)0.06 (9)74.2 (221)23.4 (894)
938.9 (652)47.5 (428)25.7 (65)61.7 (142)2.4 (182)11.7 (450)3.4 (405)81.6 (261)88.5 (491)
1110 (527)17 (271)25 (54)43 (127)6.5 (73)11.7 (696)11.4 (378)98.8 (349)70 (837)
1249.6 (462)58.7 (407)20 (48)9.1 (52)1.8 (108)3.8 (346)16 (1122)99.4 (109)38.5 (585)
1334.3 (472)52.6 (392)66 (55)ND2.7 (377)11.1 (1028)5.2 (1073)98.8 (39)67.4 (166)
1450 (497)36.8 (—)18.3 (49)18.9 (139)13.9 (26)5 (30)5.6 (232)98.2 (—)64.3 (—)
1522.5 (975)46.9 (—)99.8 (180)15.1 (48)23.7 (41)28.5 (63)42 (102)99.9 (—)76.3 (—)
Patients (n = 10)31.7 (532 ± 192)44 (280 ± 117)40.5 (—)22.3 (99.4 ± 38)6.7 (135 ± 138)13.4 (414 ± 351)9.4 (616 ± 512)85.5 (180 ± 107)58.9 (457 ± 297)
Donors (n = 7)24.1 (297 ± 27)46.3 (502 ± 5)24.4 (60 ± 27)33 (76 ± 71)6.6 (101 ± 4)15.8 (367 ± 343)6.4 (319 ± 441))88.9 (234 ± 77)76 (298 ± 68)
  • a Expression of different markers on expanded Vδ2 T cells from 10 cultures derived from MRCC patients’ PBMCs and 7 cultures derived from donors. Results are expressed in percentage of positive cells gated on the CD3+Vδ2+ population. Numbers in parentheses correspond to the mean fluorescence intensity values. For donors and patients, we have represented the mean percentage of expression and the mean fluorescence intensity values.