Table II.

Flow cytometric analysis of NOD-scid IL2rγnull spleen cellsa

Surface Markers% of Positively Stained Cells in Spleen
scid (10)bscid IL2rγnull (9)b
CD3+/CD4+0.2 ± 0.10.1 ± 0.0b
CD3+/CD8+0.3 ± 0.20.1 ± 0.0
B220+/Igκ+0.0 ± 0.00.0 ± 0.0
B220+/Igκ10.5 ± 0.56.1 ± 0.3c
Gr-1+/Mac-1+13.6 ± 2.727.9 ± 4.9b
Gr-1/Mac-1+18.5 ± 3.217.6 ± 1.6
Ter 119+17.5 ± 3.420.0 ± 1.9
LGL+/CD122+6.2 ± 0.60.2 ± 0.2c
LGL+/DX5+7.1 ± 0.70.0 ± 0.0c
H-2Kd+99.3 ± 0.398.2 ± 1.2
H-2Kd+/I-Ag7+10.1 ± 0.47.8 ± 0.5
  • a Data are expressed as mean percent ± SEM.

  • b Numbers in parentheses, number of mice.

  • c NOD-scid IL2rγnull vs NOD-scid (p < 0.05).

  • d NOD-scid IL2rγnull vs NOD-scid (p < 0.001).