Table VII.

APM component down-regulation in human DC coincubated with tumor cell linesa

APM Component% Positive Cells
Coincubation with PCI-4B cellsCoincubation with PCI-38 cells
Donor 1Donor 2Donor 3Donor 4Donor 5Donor 6
+Tu−Tu+Tu−Tu+Tu−Tu+Tu−Tu+Tu−Tu+Tu−Tu
MB-126048614092247448603492
LMP-2687543615382134958756382
LMP-7297425352438122642741538
LMP-10497368801038455946731738
ERp57655736752575ND6042582575
  • a Monocyte-derived DC were cultured in the presence of IL-4 and GM-CSF and then coincubated with tumor cells in wells of Transwell plates as described in Materials and Methods. after coincubation for 24 h at 37°C, the DC were harvested and stained with mAbs specific for the individual APM components. The percentage of stained cells was determined by flow cytometry.