Table II.

Reproducibility and sensitivity of intracytoplasmic staining for APM components in microwave-treated or saponin-permeabilized DCa

APM Components% Positive Cells
0.25% saponinMicrowaveRange
Lab 1Lab 2
IgG control2111–2
Delta79806939–95
MB-13718126–59
LMP-256796926–84
LMP-711432–23
TAP-117963–33
ERp57161173–33
Tapasin27584210–64
  • a Comparisons were performed in two laboratories, using the same DC preparations. DC were permeabilized by microwave or saponin treatments and stained with the mAbs specific for the individual APM components. The percentage of stained cells was determined by flow cytometry. Data are mean percentages of stained cells from three cell preparations.