Table I.

Oligonucleotide primers used for the generation of recombinant immunoreceptors

1-CD28 BamHI-sense: 5′-CTGGATCCCAAATTTTGGGTGCTGGTGGTGGTTG-3′a
2-CD28-XhoI-antisense: 5′-CTACTCGAGGATCAGGAGCGATAGGCTGCGAA-3′
3-CD28-CD3ζ-antisense: 5′-GTCTGCGCTCCTGCTGAACTTCACTCTCAGGGAGCGATAGGCTGCGAAGTC-3′b
4-CD28-CD3ζ-sense: 5′-CCTATCGCTCCCTGAGAGTGAAGTTCAGCAGGAGC-3′b
5-CDζ-XhoI-antisense: 5′-CTACTCGAGGATTAGCGAGGGGGCAGGGC-3′
6-Lκ-NcoI-sense: 5′-CTACGTACCATGGATTTTCAGGTGCAGATTTTC-3′
7-hIgG1Fc-BglII-antisense: 5′-CCCACCCAGATCTTTTTTACCCAGAGACAGGGAGAG-GCTCTTCTG-3′
  • a Restriction sites are underlined.

  • b Overlapping sequences are in boldface.