Table I.

Oligonucleotide primers used for PCR and RT-PCR

NameSequence (5′–3′)GeneComments
GASM15′-TATT(G/C)GCTTAGAAAATTAA-3′ emm18 26
GASM25′-GCAAGTTCTTCAGCTTGTTT-3′ emm18 26
GASN15′-CTCACAGCGGCAGCCCTTACT-3′ enn18 −283/−262a
GASN25′-GTCTATTGCACCGTTACAAA-3′ enn18 1115–1135a
Enn18FB5′-CGGCTACTTGGATCCACAAG-3′ enn18 226/246a/BamHI site
Enn18RB5′-GGTAATTGGGATCCCATTCC-3′ enn18 977/997a/BamHI site
NF5′-AGAAGAAATCGGCGGCT-3′ enn18 214/231a
NR25′-TGGGTTGGCTGCTTCACCTG-3′ enn18 1002/1022a
  • a Nucleotide position relative to the start codon of S. pyogenes enn18 gene (accession number X80494).