Table II.

Activation of STAT5 following IL-5 treatment of blood and BAL eosinophils

SubjectsaIL-5Rα (MCF)bIL-5-Stimulated STAT5 Phosphorylationc
BlooddBALeBlooddBALe
14321641.020.31
2292800.26< background
12372320.73f0.10f
13396680.41< background
  • a Characteristics of the subjects used for these studies can be found in Table I.

  • b IL-5Rα was evaluated by flow cytometry for the eosinophils in whole blood or unpurified BAL fluid cells and reported as the median channel fluorescence (MCF) for 104 events.

  • c STAT5 phosphorylation was analyzed, after stimulation of purified blood or BAL eosinophils with 300 pM IL-5 for 15 min, by immunoblotting with anti-phosphotyrosine 694 STAT5. Immunoblot bands were quantified by densitometry, background was subtracted, and the values were normalized to the signal from the anti-STAT5 immunoblot of the same membrane. These are arbitrary units.

  • d Results are reported for the analysis of peripheral blood eosinophils acquired 48 h after segmental bronchoprovocation with Ag.

  • e Results are reported for the analysis of airway eosinophils acquired from BAL fluid 48 h after segmental bronchoprovocation with Ag.

  • f The immunoblots from which these values were derived are pictured in Fig. 4C, lanes 2 and 4. The data are normalized to the signal of the same lanes reprobed with anti-STAT5 antisera (Fig. 4D, lanes 2 and 4).