Table II.

Effects of AD on splenic T cell expression of various receptors and cytokines involved in T cell activation and costimulatory signalinga

TreatmentPercent Marker-Positive CD3+ CellsbExpression Intensity of Marker-Positive CD3+ Cells (Geometrical Mean Fluorescence Intensity)c
SxdCxSxCx
TCRβ+97.5 ± 0.197.0 ± 0.1111.6 ± 1.7109.6 ± 0.8
TCRγ/δ+7.4 ± 0.47.9 ± 0.652.3 ± 1.455.0 ± 1.2
CD28+76.6 ± 2.771.7 ± 4.637.4 ± 3.536.5 ± 2.3
CD25+10.9 ± 0.611.5 ± 0.543.4 ± 0.242.4 ± 0.5
OX40+5.9 ± 0.36.9 ± 0.316.3 ± 1.816.4 ± 1.7
CD40L+3.2 ± 0.63.9 ± 0.846.1 ± 4.446.7 ± 3.8
CTLA-4+14.7 ± 0.116.6 ± 0.155.8 ± 0.654.2 ± 0.7
4-1BB+4.9 ± 0.24.8 ± 0.356.5 ± 1.357.6 ± 3.3
IL-2+4.1 ± 0.74.6 ± 0.847.2 ± 5.348.4 ± 5.3
IFNγ+4.2 ± 0.64.6 ± 0.768.6 ± 5.163.3 ± 4.0
IL-4+0000
  • a Samples of splenocytes were analyzed by flow cytometry, gated on CD3+ lymphocytes.

  • b Values represent mean percentage or

  • c mean expression intensity (geometrical mean fluorescence intensity) of Ag receptor or cytokine-positive cells among CD3+ lymphocytes, ± SEM. Data were generated from two independent experiments.

  • d Cohorts of five 8- to 12-wk-old mice were Sx or Cx 7 days prior to study.