Table I.

Effects of AD on lymphocyte levels in peripheral lymph nodes

StrainTreatmentNumber of Cells × 106/Lymph Nodea
CD45+CD45R/B220+CD3+CD4+CD8+
Wild-type C57BL/6Sxb3.08 ± 0.56c1.06 ± 0.241.72 ± 0.341.11 ± 0.250.88 ± 0.19
Cx8.91 ± 1.78∗3.37 ± 0.84∗4.81 ± 0.96∗3.27 ± 0.74∗2.34 ± 0.55∗
μMTSx1.93 ± 0.6201.90 ± 0.621.11 ± 0.370.80 ± 0.25
Cx5.37 ± 0.50∗05.29 ± 0.49∗3.31 ± 0.32∗2.01 ± 0.18∗
CD28−/−Sx3.14 ± 0.460.60 ± 0.061.42 ± 0.060.92 ± 0.050.62 ± 0.02
Cx6.67 ± 0.90∗2.18 ± 0.11∗5.33 ± 0.32∗3.72 ± 0.22∗2.16 ± 0.12∗
Tx C57BL/6dSx2.25 ± 0.331.20 ± 0.201.06 ± 0.140.46 ± 0.060.59 ± 0.08
Cx3.53 ± 0.752.18 ± 0.52∗1.27 ± 0.290.48 ± 0.080.78 ± 0.20
  • a Absolute numbers of CD45+, B220+, CD3+, CD3+, CD4+, CD8, CD4+, and CD3+ CD4+, CD8+, (CD8+) cells per single lymph node were determined, as described in Materials and Methods.

  • b Cohorts of five 8- to 12-wk-old mice were Sx or Cx 4 wk prior to study.

  • c Values represent mean numbers of marker-positive cells per lymph node, ± SEM generated from experiments repeated three times. ∗, Denotes a significant difference (p ≤ 0.05) in values for Sx vs Cx mice.

  • d Mice were thymectomized (Tx) at 8 wk of age. Sham castration or castration was performed at 10 wk of age.