Table I.

Phenotype analysis of DCs matured in the presence of T cell subsetsa

Cell-Cell Contact CoculturesTranswell Cocultures
DC (medium)DC (IL-4, GM-CSF)DC (CD4+CD25)DC (CD4+CD25+)DC (CD4+CD25)DC (CD4+CD25+)
CD1a92 ± 4 (50 ± 30)94 ± 2 (59 ± 10)90 ± 3 (43 ± 15)89 ± 4 (47 ± 3)87 ± 5 (30 ± 11)79 ± 2 (23 ± 12)
CD8312 ± 0 (35 ± 9)54 ± 6 (48 ± 25)75 ± 8 (38 ± 16)33 ± 10b (34 ± 10)31 ± 7 (6 ± 0)27 ± 3 (7 ± 1)
CD8672 ± 5 (16 ± 2)70 ± 10 (23 ± 11)87 ± 4 (48 ± 20)48 ± 7b (31 ± 8)78 ± 3 (23 ± 3)78 ± 8b (16 ± 1)
CD8040 ± 7 (20 ± 3)78 ± 8 (33 ± 10)83 ± 9 (48 ± 6)63 ± 12 (32 ± 4)38 ± 3 (13 ± 2)26 ± 6 (9 ± 1)
CD4096 ± 02 (314 ± 37)95 ± 2 (574 ± 18)97 ± 1 (656 ± 39)95 ± 2 (368 ± 79)b99 ± 1 (59 ± 2)96 ± 1 (42 ± 1)b
HLA-DR97 ± 1 (223 ± 40)96 ± 1 (259 ± 44)98 ± 1 (243 ± 46)94 ± 2 (181 ± 32)93 ± 2 (93 ± 25)96 ± 4 (73 ± 23)
CD9571 ± 9 (71 ± 6)71 ± 6 (50 ± 25)84 ± 5 (68 ± 23)69 ± 9 (65 ± 14)3 ± 2 (21 ± 4)2 ± 1 (14 ± 2)
  • a imDCs of 5 days were cultured in contact and in Transwell with CD4+CD25 and CD4+CD25+ T cell subsets. DCs in control cultures were either left in medium alone or supplemented with IL-4 and GM-CSF. Results are represented as mean ± SE of percentage/MFI of cells positive for the indicated markers. MFI are indicated in parentheses. Results are from four of the cell-cell contact and two of the Transwell experiments. Statistical significance, as determined by nonparametric Mann-Whitney, is indicated.

  • b , p < 0.05. The indicated p values were obtained on comparison of DC (CD4+CD25) and DC (CD4+CD25+) and on comparison of DC (CD4+CD25+) in cell-cell contact and Transwell cultures.