lpg2− parasites induce comparable early inflammatory and proliferative responses as WT L. majora
| Cells/LN ± SE (×106) | Proliferation ± SE (×103 cpm) | |||
|---|---|---|---|---|
| Medium | SLA | Anti-CD3 | ||
| Uninfected | 1.9 ± 0.2 | 0.2 ± 0.5 | 0.3 ± 0.1 | 57.1 ± 12.6 |
| WT | 14.6 ± 0.5 | 8.5 ± 1.5 | 16.7 ± 1.9 | 39.7 ± 1.2 |
| lpg2 − | 17.0 ± 3.8 | 25.6 ± 3.7 | 21.3 ± 3.7 | 50.2 ± 1.30 |
| lpg2−/+ LPG2 | 14.0 ± 4.0 | 25.8 ± 10.8 | 28.4 ± 4.7 | 65.3 ± 18.3 |
a Naive (uninfected) mice or BALB/c mice infected for 3 days with WT, lpg2, or lpg2−/LPG2 L. major were sacrificed and the total number of cells in the draining popliteal LNs were enumerated. Cells were stimulated with SLA or anti-CD3/anti-CD28 for 3 days and pulsed with [3H]thymidine for 18 h, and cellular proliferation was measured by counting the amount of radioactivity incorporated by the cells.