Table I.

FACS analysis of cell populations in CD93−/− and wild-type micea

CompartmentCell MarkerPredominant Cell TypeCD93−/− MiceWild-Type Mice
Bone marrowCD43+B220+Pro B11.5 ± 1.711.0 ± 2.6
B220+IgMPre B28.4 ± 4.332.0 ± 7.4
IgM+IgDImmature B15.2 ± 6.517.5 ± 7.0
IgM+IgD+Mature B8.7 ± 1.98.5 ± 1.2
7/4+Neutrophils20.2 ± 9.916.9 ± 11.0
ThymusCD4+/CD8+T62.6 ± 3.353.3 ± 8.8
CD4+T21.8 ± 5.724.2 ± 16.2
CD8+T8.6 ± 3.414.4 ± 11.1
SpleenB220+B cells46.9 ± 10.150.6 ± 10.6
CD4+T20.3 ± 6.021.3 ± 5.4
CD8+T9.6 ± 4.09.8 ± 3.5
F4/80+Mac/Eos/DC4.6 ± 2.64.4 ± 3.0
7/4+Neutrophils7.9 ± 3.58.6 ± 5.2
DX-5+NK/T5.4 ± 0.54.9 ± 1.2
Lymph nodesB220+B cells29.6 ± 5.928.6 ± 12.8
CD4+T47.3 ± 4.851.6 ± 11.1
CD8+T17.1 ± 6.015.0 ± 6.2
Peripheral bloodB220+B cells29.2 ± 8.025.8 ± 12.5
CD90.2+T cells26.5 ± 8.422.2 ± 7.9
F4/80+Monocyte/Eos6.8 ± 2.76.3 ± 3.5
7/4+Neutrophils25.1 ± 7.232.2 ± 12.7
PeritoneumB220+B cells26.4 ± 8.317.7 ± 3.9
CD90.2+T cells9.7 ± 3.211.4 ± 5.5
F4/80+Macrophages35.3 ± 11.035.7 ± 6.8
7/4+Neutrophils4.3 ± 1.34.9 ± 1.4
  • a Cellular composition of CD93−/− and control bone marrow, thymus, spleen, lymph nodes, peripheral blood, and peritoneum. Four to eight mice of each strain were examined at 3 mo of age. For all compartments, the percentages shown represent the percentages of all leukocytes present in that compartment. Total cell counts in all tissues were the same in CD93−/− and wild-type mice. Shown values represent mean ± SEM.