PCR primers used in this study and length of the amplified gene and competitor fragments. Only a noncompetitive PCR was used for IL-12p40
| Amplified Target Size (bp) | Competitor Size (bp) | Primer Sequences | |
|---|---|---|---|
| iNOS | 497 | 580 | Sense: 5′-CCC TTC CGA AGT TTC TGG CAG CAG CAG C-3′ |
| Antisense: 5′-GGC TGT CAG AGC CTC GTG GCT TTG G-3′ | |||
| IFN-γ | 365 | 500 | Sense: TGG ACC TGT GGG TTG TTG ACC TCA AAC TTG GC-3′ |
| Antisense: 5′-TCG ATC TTG GCT TTG CAG CTC TTC CTC ATG GC-3′ | |||
| IL-12-p40 | 452 | Sense: 5′-CGT GCT CAT GGC TGG TGC AAA G-3′ | |
| Antisense: 5′-CTT CAT CTG CAA GTT CTT GGG C-3′ | |||
| β-Actin | 540 | 400 | Sense: 5′-GTG GGC CGC TCT AGG CAC CAA-3′ |
| Antisense: 5′-CTC TTT GAT GTC ACG CAC GAT TTC-3′ |