Table I.

PCR primers used in this study and length of the amplified gene and competitor fragments. Only a noncompetitive PCR was used for IL-12p40

Amplified Target Size (bp)Competitor Size (bp)Primer Sequences
iNOS497580Sense: 5′-CCC TTC CGA AGT TTC TGG CAG CAG CAG C-3′
Antisense: 5′-GGC TGT CAG AGC CTC GTG GCT TTG G-3′
IFN-γ365500Sense: TGG ACC TGT GGG TTG TTG ACC TCA AAC TTG GC-3′
Antisense: 5′-TCG ATC TTG GCT TTG CAG CTC TTC CTC ATG GC-3′
IL-12-p40452Sense: 5′-CGT GCT CAT GGC TGG TGC AAA G-3′
Antisense: 5′-CTT CAT CTG CAA GTT CTT GGG C-3′
β-Actin540400Sense: 5′-GTG GGC CGC TCT AGG CAC CAA-3′
Antisense: 5′-CTC TTT GAT GTC ACG CAC GAT TTC-3′