Table I.

Chemokine expression during PMN transendothelial migration to intact bacteria or purified LPSa

IL-8 (pg/ml)MCP-1 (pg/ml)RANTES (pg/ml)IL-6 (pg/ml)GRO-α (pg/ml)
S. pneumoniae 430 (±14)127 (±6.1)762 (±5.2)12 (±1.5)<15
S. aureus 353 (±16)39 (±5.1)346 (±3.5)<12<15
E. coli 477 (±13)164 (±12.1)680 (±11.1)48 (±2.1)<15
Purified LPS1037 (±87)288 (±36.3)Not tested482 (±16.4)1933 (±31.6)
  • a Supernatants were collected from the abluminal surface of the endothelial monolayer at the completion of the migration experiments and filtered to remove bacteria and PMNs. Chemokine expression was assayed by standard ELISA techniques. n = 4 experiments.