Table II.

Effect of degranulation on procaspase-3 and procaspase-4 levels

CellsCaspase-3Caspase-4
Expt. 1Expt. 2Expt. 3MeandExpt. 1Expt. 2Expt. 3Meand
Immature HMC-1
Controla23.6 ± 0.539.9 ± 0.839.9 ± 0.434.534.3 ± 0.826.4 ± 0.730.2 ± 0.630.3
+48/80b15.5 ± 0.3e11.6 ± 0.3e14.8 ± 0.3e14.025.8 ± 0.7e6.5 ± 0.2e16.4 ± 0.6e16.2
n c 347355321340273219
Mature HMC-1
Controla20.2 ± 0.346.4 ± 1.252.0 ± 0.939.636.3 ± 1.237.0 ± 1.446.4 ± 1.139.9
+48/80b15.8 ± 0.2e17.1 ± 0.5e17.7 ± 0.9e16.87.6 ± 0.4e17.0 ± 0.5e14.7 ± 0.5e13.1
n c 484283109272121213
RPMC
Controla42.0 ± 1.538.3 ± 1.141.6 ± 0.740.628.1 ± 0.712.5 ± 0.520.3
+48/80b27.9 ± 1.6e27.1 ± 1.6e14.2 ± 0.4e23.16.0 ± 0.3e10.4 ± 0.5e8.2
n c 144190224233128
  • a Mean procaspase fluorescence of untreated cells for individual experiments ± SEM (GSU).

  • b Mean procaspase fluorescence of cells treated for 18 h with 1 μg/ml compound 48/80 for individual experiments ± SEM (GSU).

  • c Total number of cells used for quantification of fluorescence.

  • d Mean of individual experiments.

  • e p < 0.005.