Table I.

Effects of treatment with activators or TPEN on Zinquin fluorescence of mast cells

Mast Cell TypeTreatmentaZinquin Fluorescenceb (% of control, ± SEM)
Immature HMC-148/80 (1 μg/ml)59.5 ± 6.1d
IgE (5 μg/ml)+ anti-IgE (0.2 μM)58.4 ± 7.1c
TPEN (50 μM)39.4 ± 7.6d
Mature HMC-148/80 (1 μg/ml)55.9 ± 2.8d
RPMC48/80 (1 μg/ml)35.2 ± 1.5d
Bone marrow derivedIgE (5 μg/ml) + anti-IgE (0.2 μM)65.8 ± 9.4c
PMA (100 nM)+ A23187 (0.5 μM)41.4 ± 27.7c
TPEN (100 μM)53.7 ± 6.8d
CBMC48/80 (1 μg/ml)49.1 ± 2.5d
  • a Cells were treated for 18 h at 37°C with reagents in culture medium, except for TPEN, which was added for 2 h.

  • b Zinquin fluorescence of cells expressed as percentage of fluorescence of control cells.

  • At least 20 cells were quantified by image analysis from replicate tubes.

  • c p < 0.05;

  • d p < 0.005.