Table II.

Combined intracellular cytokine data from all day 20 experiments performeda

Expt.In Vivo TreatmentPercentage of Thy1.1 Cells Staining Positive
IFN-γTNF
1Transfer only0.0 ± 0.012.0 ± 2.0
LPS1.6 ± 0.838.1 ± 2.6
Peptide/IgG/LPS20.4 ± 4.249.0 ± 3.7
Peptide/anti-CD40/LPS16.5 ± 5.425.5 ± 3.3
2Peptide/IgG/LPS9.6 ± 0.653.7 ± 1.1
3Peptide/IgG/LPS19.9 ± 1.451.5 ± 8.1
Peptide/anti-CD40/LPS48.1 ± 4.765.9 ± 2.2
4Peptide/IgG19.5 ± 4.138.2 ± 4.3
Peptide/anti-CD40/LPS68.2 ± 4.374.1 ± 1.5
5Peptide/IgG/LPS27.6 ± 9.538.4 ± 2.9
Peptide/anti-CD40/LPS24.0 ± 3.840.2 ± 1.1
6Peptide/anti-CD40/LPS56.3 ± 1.380.3 ± 1.4
  • a For each experiment, only the treated mice that had substantial CD4+Thy1.1+ T cell populations on day 20 available for restimulation in triplicate are listed. Experiment numbers correspond to the same experiments in Table I. The percentage of Thy1.1 cells that stained positive for IFN-γ or TNF are listed as the average ± SEM from triplicate cultures.