Table I.

Combined absolute number data from all day 20 experiments performeda

Expt.Experimental ConditionsNo. of CD4 Cells Expressing Thy1.1 (×10−3)
Transferred cell no. (×10−6)Peptide dose (μg)LPS dose (μg)CD40 dose (μg)Inguinal LNAxillary LNBrachial LNMesenteric LNSpleenLiverLung
12.00.690.430.952.8615.131.230.04
2.0501.421.101.642.7037.201.170.35
2.01000.390.130.290.83<4.730.630.06
2.0100250<0.400.340.450.446.890.500.10
2.0100503.882.524.0731.58406.8523.0610.65
2.010050250NDbNDNDNDNDNDND
2.0100752500.500.320.281.5022.911.530.07
22.0<0.18<0.08<0.16<0.18<6.47<0.42<0.04
2.0252500.600.290.367.6830.391.210.13
2.0100<0.24<0.11<0.25<0.266.78<0.72<0.06
2.0100250<0.40<0.10<0.21<0.31<10.36<0.33<0.12
2.0100253.262.302.408.86175.465.151.45
2.010025250NDNDNDNDNDNDND
32.01000.260.400.130.9917.582.150.34
2.0100250<0.37<0.30<0.36<0.27<8.76<0.50<0.09
2.0100100.240.280.190.6265.155.022.29
2.010010250NDNDNDNDNDNDND
40.50.32<0.18<0.210.088.020.580.14
0.5102500.520.530.110.7236.41<0.560.27
0.51000.270.18<0.210.2519.110.680.17
0.51002500.470.150.340.1727.350.960.10
0.5100100.180.180.280.6669.756.450.41
0.5100102502.731.721.028.93613.7159.215.18
50.5<0.31<0.15<0.36<0.67<5.82<0.57<0.12
0.510250<0.30<0.28<0.330.508.060.59<0.25
0.51000.16<0.18<0.180.294.350.720.11
0.51002500.38<0.23<0.23<0.456.700.620.17
0.5100100.470.260.290.3125.741.770.57
0.5100102500.420.410.133.7437.151.310.32
60.50.22<0.12<0.230.57<8.42<0.63<0.09
0.5102500.390.060.140.227.870.460.21
0.5100<0.15<0.12<0.27<0.766.200.70<0.12
0.5100250<0.14<0.04<0.18<0.70<7.44<0.47<0.05
0.510010<0.33<0.14<0.16<0.62<5.440.490.09
0.5100102501.500.300.9713.40272.5412.562.67
  • a Listed are the number of cells adoptively transferred and the doses of peptide, anti-CD40 mAb, and LPS injected. Results list the number (×10−3) of CD4+Thy1.1+ cells present in each tissue examined from six separate experiments. Numbers were obtained as described in the legend to Fig. 8b.

  • b ND, No data was obtained.