Table II.

Expansion of IgA, but not IgG anti-TT ASC in B6-hbcl-2 Tg micea

MiceImmunizationIgG anti-TT ASCIgA anti-TT ASC
Non-TgPBS41.4 ± 7.914.3 ± 3.8
Non-TgTT-Al(OH)33970.8 ± 207.167.2 ± 10.1
bcl-2-TgPBS60.8 ± 16.130.4 ± 12.4
bcl-2-TgTT-Al(OH)34561.4 ± 335.61033.7 ± 298.6
  • a Eight-week-old B6-hbcl-2 Tg and non-Tg mice were immunized l.p. with 3 μg of TT-Al(OH)3. Animals were boosted with the same dose of Ag 15 days later. ASC were measured by ELISPOT in the spleen 21 days after secondary immunization. Results are expressed as the mean number of ASC ± SD in the total spleen (4 animals/group).