Table VIII.

Exonuclease activity in the VHD or DJH junctions

Excision SitePeripheral B CellsTonsilar Plasma CellsIgG and IgM CellsMean
NonproductiveVH 3′2.2 ± 0.42.2 ± 0.81.1 ± 0.62.1 ± 0.3ab
D 5′4.6 ± 0.64.8 ± 2.53.2 ± 1.14.5 ± 0.5ab
D 3′4.7 ± 0.73.5 ± 1.78.7 ± 3.15.0 ± 0.6a
JH 5′6.5 ± 0.77.5 ± 1.27.6 ± 1.66.8 ± 0.6ab
ProductiveVH 3′1.9 ± 0.11.5 ± 0.21.6 ± 0.21.8 ± 0.1ab
D 5′4.5 ± 0.3c4.9 ± 0.45.3 ± 0.54.7 ± 0.2ab
D 3′5.5 ± 0.35.2 ± 0.55.3 ± 0.55.4 ± 0.2a
JH 5′6.0 ± 0.35.9 ± 0.45.6 ± 0.45.9 ± 0.2ab
  • a Significant (p < 0.05) differences between the excision in the VH coding end and the other coding ends.

  • b Significant (p < 0.05) differences between the excision in the D5′ coding end and the other coding ends.

  • c One rearrangement (Z80724) had a D region containing 11 5′ consecutive matches followed by one mismatch and 11 3′ consecutive matches. Both consecutive matching segments were identified as IGHD3–22*01. For analysis, the 3′ 11 consecutive match was assigned as the D segment and the 5′ 11 consecutive match was used only to identify the VH-D junction.