Table VII.

TdT activity and frequency of microhomology in the VH-D or D-JH junctions of nonproductive and productive rearrangements

NonproductiveProductive
N addition (bp)No N addition (% of total)Microhomology (% of total)N addition (bp)No N addition (% of total)Microhomology (% of total)
VH-DD-JHVH-DD-JHVH-DD-JHVH-DD-JHVH-DD-JHVH-DD-JH
Peripheral B cells11.0 ± 1.1a9.5 ± 1.14.6a9.2b2.3a4.6b7.8 ± 0.3c7.3 ± 0.41.88.8b0.52.8b
Tonsilar plasma cells7.0 ± 2.98.5 ± 1.20a0a0a0a6.7 ± 0.56.6 ± 0.64.35.72.82.8
IgG and IgM cells6.3 ± 1.48.5 ± 2.00a0a0a0a8.0 ± 0.77.0 ± 0.64.84.83.64.8
Mean10.2 ± 1.0a9.2 ± 0.9a3.67.21.83.6b7.7 ± 0.37.1 ± 0.33.07.3b1.63.2b
  • a Significant (p < 0.05) differences between nonproductive and productive rearrangements.

  • b Significant (p < 0.05) differences between the VHD and DJH junctions.

  • c One rearrangement (Z80724) had a D region containing 11 5′ consecutive matches followed by one mismatch and 11 3′ consecutive matches. Both consecutive matching segments were identified as IGHD3–22*01. The 3′ 11 consecutive match was assigned as the D segment. The 5′ 11 consecutive match was considered as a match only for the purpose of identifying the VH-D junction and analyzing junctional diversity in this region.