Table VI.

D segment reading frame usea

D GeneRF1Frequency of RF1 UsageRF2Frequency of RF2 UsageRF3Frequency of RF3 Usage
NPPNPPNPP
1-1GTTGT00VQLER00YNWND01 (100)bc
1-7GITGT04.5 (53)bcV*LEL1 (100)0YNWNY04 (47)c
1-14GITGT00V*PEP00YNRNH00
1-20GITGT02.5 (26)V*LER00YNWND07 (74)bc
1-26GIVGAT2 (100)bc10 (32)V*WELL03 (10)YSGSYY018 (58)bc
2-2RIL**YQLLC3 (23)c0GYCSSTSCYA5 (8)17 (68)bDIVVVPAAM5 (38)8 (32)
2-8RILY*WCMLY1 (50)0GYCTNGVCYT04 (80)bcDIVLMVYAI1 (50)1 (20)
2-15RIL*WW*LLL1 (33)c0GYCSGGSCYS1 (33)13 (81)bcDIVVVVAAT1 (33)3 (19)
2-21SILWW*LLF2 (40)c0AYCGGDCYS1 (20)1 (25)bcHIVVVIAI2 (40)3 (75)b
3-3VLRFLEWLLY1 (33)10 (29)YYDFWSGYYT2 (66)14 (41)ITIFGVVII1 (33)10 (29)
3-9VLRYFDWLL*05 (33)YYDILTGYYN010 (66)bcITIF*LVII2 (100)0
3-10VLLWFGELL*2 (40)10 (24)YYYGSGSYYN1 (20)21 (51)bcITMVRGVII2 (40)10 (24)
3-16VL*LRLGELCLY01 (13)YYDYVWGSYAYT07 (88)bcIMITFGGVMLI00
3-22VLL***WLLL4 (50)0YYYDSSGYYY3 (38)40 (87)bcITMIVVVIT1 (13)6 (13)
4-4/4-11*LQ*L00DYSNY02 (50)cTTVT02 (50)bc
4-17*LR*L2 (50)0DYGDY2 (50)b11 (52)bTTVT010 (48)c
4-23*LRW*L00DYGGNS03 (60)bcTTVVT02 (40)c
5-5/5-18VDTAMV06 (50)WIQLWL01 (9)GYSYGY1 (100)5 (42)
5-12VDIVATI1 (50)3 (27)WI*WLRL01 (9)GYSGYDY1 (50)7 (64)b
5-24VEMATI1 (100)3 (50)*RWLQL01 (17)RDGYNY02 (33)bc
6-6EYSSSS1 (100)4 (44)SIAAR05 (56)bV*QLV00
6-13GYSSSWY013 (42)GIAAAG2 (100)b14 (45)V*QQLV04 (13)
6-19GYSSGWY1 (33)15 (47)GIAVAG2 (66)b12 (38)V*QWLV05 (16)
6-25GYSSGY00GIAAA00V*QRL00
7-27LTG02 (40)*LG00NWG03 (60)b
  • a Columns 2, 5, and 8 show the amino acid translation of each D segment for each RF followed by the number of times a nonproductive or productive rearrangement using a specific D segment uses the indicated RF. The number in parentheses indicates the percentages with which rearrangements using the specific D segment use the given reading frame. The asterisk (*) represents stop codons; hydrophilic amino acid residues are italicized; hydrophobic amino acid residues are black. Amino acids are defined as hydrophilic or hydrophobic as described (38 ).

  • b Significantly (p < 0.05) higher frequency when compared to the expected random frequency.

  • c Significantly (p < 0.05) higher frequency between nonproductive and productive.