Table I.

WT, but not KARAP/DAP12−/− BM-DC up-regulate CD86 molecules in coculture with WT NK cellsa

BM-DC WTBM-DC KARAP-DAP12−/−
0+NK0+NK
CD86%82 ± 287 ± 274 ± 375 ± 3
MFIc677 ± 94821 ± 102b605 ± 74655 ± 74
CD80%62 ± 780 ± 948 ± 1271 ± 10
MFI107 ± 53156 ± 73132 ± 64167 ± 86
CD40%56 ± 860 ± 245 ± 558 ± 2
MFI76 ± 1677 ± 1866 ± 1572 ± 12
I-Ab%87 ± 287 ± 181 ± 383 ± 3
MFI169 ± 24168 ± 27139 ± 13152 ± 22
  • a Spleen-derived NK cells of SCID mice were negatively selected using the Spin Sep kit (StemCell Technologies), then stimulated overnight in 1000 IU/ml IL-2 and incubated (or not) with BM-DC (GM/IL-4) WT vs KARAP-DAP12 knockin for 24 h at a ratio of 1 DC to 2 NK cells. After coculture or incubation alone, FACS analyses gating on CD11c+ cells were performed using anti-I-Ab, CD40, CD80, and CD86 mAb. Mean ± SD of three independent experiments are shown for each individual molecular pattern, Student’s t test was used to compare expression of molecules on BM-DC alone vs BM-DC after coculture with NK cells.

  • b p < 0.05.

  • c MFI, mean fluorescence intensity.