Table II.

Kinetic parameters of glycolipid Ag analogue/TCR interactions

T CellKd (nM) ± SEM (n)at1/2 (min at 37°C)b ± SEM (n)Hill Coefficient (h) ± SEM (n)
αGalCercOCH3,4DαGalCercOCHαGalCercOCH
iNKT hybridomas
N37-1H5a7.5 ± 0.3 (3)29.9 ± 0.3 (2)ND10.7 ± 3.0 (4)ND2.8 ± 0.4 (3)2.2 ± 0.4 (2)
N38-2C128.5 ± 2.0 (4)22.1 ± 1.7 (2)ND42.1 ± 4.3 (5)ND2.8 ± 0.3 (3)2.3 ± 0.4 (2)
C57BL/6 iNKT cells
Thymic
TCRβ+10.7 ± 3.0 (4)31.2 ± 0.4 (2)59.5 ± 3.1 (2)9.8 ± 0.9 (5)13.0 ± 1.9 (2)2.5 ± 0.2 (3)ND
Vb8.1,8.2+10.5 ± 0.1 (2)35.7 ± 1.9 (2)
Vb7+16.0 ± 0.8 (2)46.6 ± 1.8 (2)
Splenic8.8 ± 0.7 (4)27.4 ± 10.9 (2)NDNDND2.7 ± 0.2 (3)ND
Splenic C57BL/6 iNKT cells expanded with glycolipid Ag stimulation (96 h)
αGalCer9.1 ± 0.4 (2)44.2 ± 2.9 (2)
OCH9.1 ± 0.6 (2)39.7 ± 7.2 (2)
3,4D6.4 ± 0.5 (2)25.1 ± 1.9 (2)
  • a n, Number of experimental values.

  • b Calculations of the kinetic parameters are described in Materials and Methods (also see Results for details).

  • c αGalCer data is the same as presented in Table 1.