Table II.

Cytokine microarray with supernatants from confluent MSCa

Growth FactorsFold Increase (positive control/unknown)
Chemokines
 RANTES, GRO-α1
 MCP-1, IL-82
 MIP-1β, SDF-1αND
Cytokines
 Broad-acting
  IL-63
 Proinflammatory
  IL-2, IL-12ND
  IFN-γ, TNF-α, IL-1α1
 Anti-inflammatory
  IL-4, IL-10, IL-13ND
  TGF-β1
 Hemopoietic
  G-CSF, GM-CSF1
Angiogenic
 Angiogenin1
 Oncostatin M1
  • a Cell-free supernatants were taken from confluent MSC of three different BM donors and then analyzed by microarray. Results are from three of the samples. The densitometric scans of the internal positive controls were normalized to 3-fold, and the unknowns calculated accordingly. Details of the procedure are described in Materials and Methods. MCP-1, monocyte chemoattractant protein 1; MIP-1β, macrophage inflammatory protein; SDF-1α, stromal-derived factor.