Table III.

Lack of interference with native melanoma reactivity

Responder TILStimulators (pg/ml IFN-γ released)
Auto Mel526 Mel624 Mel888 Mel938 Mel
No vector3,509 (129)380 (33)550 (50)641 (13)561 (48)
YFP3,269 (178)209 (31)316 (8)411 (206)336 (65)
APB3,369 (132)3,930 (257)2,579 (193)310 (33)282 (23)
Stimulators (pg/ml GM-CSF released)
No vector1,190 (102)19 (3)66 (13)120 (9)67 (15)
YFP1,325 (143)15 (14)54 (10)88 (12)45 (1)
APB1,795 (192)995 (86)841 (186)31 (14)8 (6)
  • ;a TIL from a melanoma patient 5 that were reactive with autologous tumor, but not with HLA-A2-matched melanoma cell lines were transduced with TCR vector APB and tested for reactivity. Responder TIL were cocultured with the HLA-A2-positive autologous tumor cell line (Auto Mel), or HLA-A2-positive cell lines 526 and 624, or HLA-A2-negative cell lines 888 and 938. Following coculture, IFN-γ and GM-CSF levels were determined in culture medium from no vector TIL, control YFP vector-transduced TIL, or TCR vector-transduced TIL (APB). Data are the mean values of quadruplicate samples, with SD in parentheses.