Table II.

Melanoma reactivity of TCR-transduced PBL;a

Responder CellsNoneStimulators (pg/ml IFN-γ released)
526 Mel (A2+)624 Mel (A2+)888 Mel (A2)938 Mel (A2)
No vector9 (12)30 (1.4)101 (91)91 (22)28 (40)
YFP8 (9.7)35 (12)56 (18)150 (75)80 (98)
APB80 (62)2,528 (305)1,614 (298)245 (100)63 (72)
BIA146 (51)1,437 (187)866 (89)247 (82)103 (134)
TIL56 (20)2,713 (717)2,240 (235)10 (14)21 (30)
  • ;a Cytokine secretion of TCR vector-transduced PBL from melanoma patient 2 (responders) was cocultured with the HLA-A2-positive cell lines 526 and 624 or HLA-A2-negative cell lines 888 and 938. Following coculture, IFN-γ levels were determined in culture medium from no vector PBL, control YFP vector-transduced PBL, or TCR vector-transduced PBL (APB and BIA). Melanoma-reactive TIL from patient 2 were used as control responder cells. Data are the mean values of quadruplicate samples, with SD in parentheses.