Table II.

Composition of leukocytic infiltrates in mutant mice at ≥200 daysa

B6.kdCD4−/− kd/kdCD8−/−kd/kdRag−/−kd/kd
CD45+Diffuse infiltrate with peritubular aggregates 61.6 ± 8.8 cells/HPFDiffuse infiltrate with peritubular aggregates 58.2 ± 7.3 cells/HPFDiffuse infiltrate with peritubular aggregates 64.5 ± 9.2 cells/HPFDiffuse infiltrate with peritubular aggregates 31.2 ± 4.6 cells/HPFb
T cells10–20% of infiltrate10–20% of infiltrate10–20% of infiltrateNegative
CD410–20% of infiltrateNegative10–20% of infiltrateNegative
CD81–5% positive10–20% of infiltrateNegativeNegative
IL-2R1–5% positive<1% positive1–5% positiveNegative
>75% leukocytes>75% leukocytes>75% leukocytes∼50% leukocytes
NK∼10% leukocytes∼10% leukocytes∼10% leukocytes∼50% leukocytes
ICOS5–10% of infiltrate∼10–20% of infiltrate1–5% of infiltrateNegative
  • a Assessed in three to four kidneys/group; HPF, high power field; CD45+ cells determined in six consecutive fields/sample (mean ± SD);

  • b , p < 0.01 vs infiltrates in each of the other three groups listed (t test); additional semiquantitative assessment <1%, 1–5%, 5–10%, 10–20%, 20–50%, 50–75%, or >75%.