Table I.

Fraction of total CD4+ tonsil T cells displaying a Th1, Th2, or Th0 phenotype, either directly after isolation (primary cells) or after in vitro activation/expansion (cell lines)a

Expt.Primary Cells (%)Cell Lines (%)
Th1Th2Th0Th1Th2Th0
10.61.30.2123121
24.23.70.35.4524.9
36.12.40.425147.8
  • a Cells were either directly analyzed or expanded and then analyzed as described in Fig 2. The classification of Th cell phenotype was based on dot-plots of IL-4 or IL-13 vs IFN-γ as shown in Fig 2. For each sample, the dot-plot displaying the largest fraction of cells being positive for IL-4 or IL-13, respectively, was used for calculations of table values.