Table I.

Activation marker profile of myeloid, lymphoid, and plasmacytoid DC

Activation Marker^a	DC Subset^b	Mean Intensity of Fluorescence^c
Activation Marker^a	DC Subset^b	Naive	p33-VLP	CpG
CD80
	CD8⁻	55	80	179
	CD8⁺	19	54	158
	Gr1⁺	6	14	186
CD86
	CD8⁻	274	396	704
	CD8⁺	123	212	743
	Gr1⁺	49	76	290
CD40
	CD8⁻	133	204	289
	CD8⁺	43	57	205
	Gr1⁺	11	15	81
MHC class II
	CD8⁻	168	281	483
	CD8⁺	81	160	547
	Gr1⁺	73	149	422

a Groups of mice were immunized s.c. with 20 μg of p33-VLPs or, alternatively, with 20 nmol of CpGs, whereas untreated mice served as controls. CD11c⁺ cells were isolated 24 h after treatment from draining lymph nodes and were assessed for the expression of CD80, CD86, CD40, and MHC class II.
b CD11c⁺ DC subtypes could be discriminated by CD8 and Gr1 staining. Myeloid DCs are CD8⁻, while DCs of lymphoid origin are CD8⁺ and plasmacytoid DC express the Gr-1 surface marker.
c Values represent the mean fluorescence intensity measured by flow cytometry. LN cells pooled from three or four mice per group were used for the analysis. At least 3.5 × 10³ events for every DC subset were acquired. One representative of two similar experiments is shown.