Table I.

Activation marker profile of myeloid, lymphoid, and plasmacytoid DC

Activation MarkeraDC SubsetbMean Intensity of Fluorescencec
Naivep33-VLPCpG
CD80
CD85580179
CD8+1954158
Gr1+614186
CD86
CD8274396704
CD8+123212743
Gr1+4976290
CD40
CD8133204289
CD8+4357205
Gr1+111581
MHC class II
CD8168281483
CD8+81160547
Gr1+73149422
  • a Groups of mice were immunized s.c. with 20 μg of p33-VLPs or, alternatively, with 20 nmol of CpGs, whereas untreated mice served as controls. CD11c+ cells were isolated 24 h after treatment from draining lymph nodes and were assessed for the expression of CD80, CD86, CD40, and MHC class II.

  • b CD11c+ DC subtypes could be discriminated by CD8 and Gr1 staining. Myeloid DCs are CD8, while DCs of lymphoid origin are CD8+ and plasmacytoid DC express the Gr-1 surface marker.

  • c Values represent the mean fluorescence intensity measured by flow cytometry. LN cells pooled from three or four mice per group were used for the analysis. At least 3.5 × 103 events for every DC subset were acquired. One representative of two similar experiments is shown.