Table I.

Generation of NK cells in vitro from bone marrow progenitors in the absence of IL-15R α chain on hematopoietic cellsa

MicebPrimary CultureSecondary CultureTotal Yieldd% NK1.1+
CytokinesYieldcCytokinesYieldc
WTIL-150.3IL-150.90.31.2
IL-7/SCF/flt3L9.5IL-7/SCF/flt3L5.956.11.5
IL-7/SCF/flt3L9.5IL-154.643.796.8
IL-15Rα−/−IL-150.1IL-150.50.10.9
IL-7/SCF/flt3L11.3IL-7/SCF/flt3L7.281.42.3
IL-7/SCF/flt3L11.3IL-155.258.895.5
  • a Sorted Lin c-kit+ cells were cultured for 5 days in the indicated primary culture cytokines. On day 5, the cells were harvested, washed, and replated in the indicated secondary culture cytokines for 6 days. On day 6, the cells were harvested, counted, and stained for NK1.1 expression.

  • b BM Lin c-kit+ cells were purified from WT or IL-15Rα−/− mice.

  • c Yield represents the fold increase in total cell number.

  • d Total yield is the product of the yield in the primary and secondary cultures.