Table I.

Mutational characteristics in VH gene segments from GCs of TCR β−/− and wild-type mice

TCR β−/−Wild-Type
VH186.2 segments
Mutation frequencya
 Total1.41.8
 CDRs2.94.6
 FRs0.91.0
R/S ratio
 CDRs61/013/1
 FRs3/13/1
Position 33b4235
Position 50c00
DFL16.1d6141
Tyr95e5174
Analogue segments
Mutation frequency
 Total0.5NA
 CDRs0.8NA
 FRs0.4NA
R/S ratio
 CDRs4/1NA
 FRs1/1NA
DFL16.1 (%)2NA
Tyr95 (%)12NA
  • a Point mutations (percentage (%) of base pairs). R, replacement; S, silent.

  • b Percentage (%) of sequences containing mutation (W → L) at codon 33.

  • c Percentage (%) of sequences containing mutation (R → G) at codon 50.

  • d Percentage (%) of rearrangements using DFL16.1 segments.

  • e Percentage (%) of rearrangements encoding tyrosine at codon 95 in CDR3. NA, not applicable.