Table II.

PGES activity in fractions from paws of AIA ratsa

TimePaw FractionPGE2 (ng/ml)
Primary pawSecondary paw
Day 1S10032 ± 3 (4)27 ± 6 (4)
P100138 ± 26 (4)32 ± 1 (4)
Day 3S10027 ± 7 (4)30 ± 6 (4)
P100152 ± 19 (3)33 ± 3 (3)
Nonenzymatic21 ± 4 (4)
  • a Soluble (S100) and microsomal membrane fractions (P100) were prepared from pulverized rat paw tissue extracts and characterized for production of PGE2. Protein samples (0.1 mg of protein/ml) were incubated with 1 μM PGH2 for 40 s in 100 mM phosphate buffer, pH 7.4, 2 mM EDTA, and 2.5 mM GSH, and the reaction was terminated with 0.1 N HCl and 1 mg/ml stannous chloride. PGE2 production was analyzed by immunoassay, and all results are reported as means ± SEM (n = 3 or 4).