Table I.

Primer and probe oligonucleotide sequences for real-time quantitative PCRa

GeneAccession No.PrimersProbe
mPGES-1AB041998Forward: 5′-GCGAACTGGGCCAGAACA-3′5′-CCCCGGAGCGAATGCGTGG-3′
Reverse: 5′-GGCCTACCTGGGCAAAATG-3′
COX-2S67722Forward: 5′-CATGATCTACCCTCCCCACG-3′5′-CCTGAGCACCTGCGGTTCGCTG-3′
Reverse: 5′-CAGACCAAAGACTTCCTGCCC-3′
mPGES-2bForward: 5′-GGCAGTGGGCGGATGA-3′5′-TGGCTGGTGCATCTCATCTCTCCCA-3′
Reverse: 5′-TCGGCAGGTGTTCGGTACA-3′
COX-1S67721Forward: 5′-TCCGTGAAGATGCGCTACC-3′5′-CCAGGTGTCCCGCCCGAAAAG-3′
Reverse: 5′-AACACCTCCTGGGCCACAG-3′
cPGEScForward: 5′-TCCTGGCCTAGGTTAACAAAGG-3′5′-GTCCACACTAAGCCAATTAAGCTTTGCCCTT-3′
Reverse: 5′-CCTCCCAGTCTTTCCAATTATTGA-3′
PGISNM_031557Forward: 5′-GACGTTTTCCGCACCTTCC-3′5′-CCAGCTGGATCTGATGCTCCCCA-3′
Reverse: 5′-ACTGACAAGGAGCCTCGAGC-3′
  • a All cDNA sequences except the cPGES and mPGES-2 sequence were obtained from the GenBank database. The Taqman hydrolysis probes are gene-specific oligonucleotides dually labeled with a 6-FAM reporter dye at the 5′ end and a Black Hole Quencher-1 quencher dye at the 3′ end.

  • b The partial cDNA sequence of rat mPGES-2 was obtained by PCR sequencing of rat expressed sequence tag GenBank BF558739.

  • c The cDNA sequences of the rat cPGES were kindly provided by L. Boulet at Merck Frosst.