Table III.

Rebound isolate AC10.44 requires CD4 for infection

Virusng p24/mla
Day 6Day 12Day 6Day 12
  • a Cultures of BC7-CCR5 (106 cells/ml) and mitogen-stimulated PBMC (5 × 106 cells/ml) were inoculated with cell-free virus and incubated overnight at 37°C. Cells were washed two times with growth medium, resuspended in 10 ml of growth medium, and incubated at 37°C. Media were replaced every 3 days. Culture fluids (125 μl) were collected at 6 and 12 days and mixed with an equal volume of 0.5% Triton X-100. Viral p24 concentrations were determined by Ag-specific ELISA (DuPont). BC7-CCR5 cells were maintained in growth medium containing puromycin (0.3 μg/ml) for stable CCR5 expression.