Table III.

Organ cytokine levels at day 7 in A/JCr mice infected with 105 C. neoformans cells

CytokineLung (pg/mg)Brain (pg/mg)Spleen (pg/mg)
CNaCN/IgG2abCN/IgG3cShamdCNCN/IgG2aCN/IgG3ShamCNCN/IgG2aCN/IgG3Sham
IL-2<D.L.e<D.L.<D.L.<D.L.<D.L.<D.L.<D.L.<D.L.<D.L.<D.L.<D.L.<D.L.
IL-4150 ± 50120 ± 50440 ± 90 (↑)f380 ± 20030 ± 25110 ± 80100 ± 60<D.L.260 ± 170240 ± 1201,670 ± 840540 ± 540
IL-64,790 ± 1,3906,550 ± 1,1008,710 ± 650 (↑)9,230 ± 2,8201,290 ± 1401,800 ± 6101,970 ± 490910 ± 3902,130 ± 3109,040 ± 4,5907,710 ± 2,000 (↑)2,660 ± 430
IL-102,640 ± 9201,930 ± 5804,280 ± 1,4804,260 ± 480230 ± 120770 ± 690130 ± 6070 ± 701,550 ± 1901,100 ± 5103,400 ± 2,170930 ± 610
IL-1244,200 ± 10,46056,600 ± 13,50071,600 ± 17,00093,200 ± 27,10017,600 ± 1,77019,600 ± 3,73025,800 ± 5,3808,060 ± 8,06026,100 ± 2,74025,500 ± 7,76085,600 ± 23,700 (↑)28,600 ± 5,890
IFN-γ2,820 ± 8104,480 ± 77011,300 ± 1,410 (↑)9,620 ± 2,530520 ± 120660 ± 1702,290 ± 550 (↑)1,920 ± 151,340 ± 180970 ± 4209,890 ± 3,060 (↑)3,830 ± 520
TNF-α4,160 ± 1,1507,800 ± 1,33015,500 ± 1,500 (↑)12,100 ± 4,0001,060 ± 852,920 ± 590 (↑)3,710 ± 670 (↑)1,980 ± 701,910 ± 1,0803,420 ± 1,6508,640 ± 1,030 (↑)2,380 ± 420
MCP-1<D.L.<D.L.<D.L.<D.L.<D.L.<D.L.<D.L.<D.L.140 ± 60210 ± 731,240 ± 610<D.L.
  • a CN, Data from group given PBS and then infected with 105 C. neoformans.

  • b CN/IgG2a, Data from group given 1 mg of IgG2a and then infected with 105 C. neoformans.

  • c CN/IgG3, Data from group given 1 mg of IgG3 and then infected with 105 C. neoformans.

  • d Sham, Data from group given only PBS and not infected with C. neoformans.

  • e D.L., Detection limit in ELISA.

  • f Arrows denote statistically significant (p < 0.05) increases (↑) or decreases (↓) in average cytokine level relative to CN group. Values in table are means and SE of three to five mice per group.