Table I.

Organ cytokine levels at day 7 in A/JCr mice infected with 107 C. neoformans cells

CytokineLung (pg/mg)Brain (pg/mg)Spleen (pg/mg)
CNaCN/IgG2abShamcCNCN/IgG2aShamCNCN/IgG2aSham
IL-2100 ± 10390 ± 13820 ± 2030 ± 850 ± 204 ± 451 ± 1770 ± 2911 ± 8
IL-4320 ± 40672 ± 189180 ± 15170 ± 20160 ± 30100 ± 20276 ± 147300 ± 33350 ± 80
IL-62,960 ± 4504,904 ± 1,2991,500 ± 240600 ± 120240 ± 50 (↓)d410 ± 80387 ± 181461 ± 122329 ± 167
IL-101,370 ± 3902,260 ± 826850 ± 22030 ± 30<D.L.e80 ± 80644 ± 436<D.L.<D.L.
IL-1269,400 ± 9,920163,000 ± 49,600 (↑)80,000 ± 9,55027,200 ± 3,53016,700 ± 2,520 (↓)24,800 ± 1,56015,000 ± 7,96024,900 ± 6,25021,100 ± 7,000
IFN-γ6,580 ± 1,3806,520 ± 1,9401,260 ± 350570 ± 90170 ± 70 (↓)130 ± 1002,180 ± 480408 ± 143 (↓)262 ± 139
TNF-α8,830 ± 1,04015,600 ± 5,10023,800 ± 14,8003,290 ± 4301,610 ± 330 (↓)4,120 ± 1,4802,027 ± 8612,623 ± 6813,390 ± 1,120
MCP-14,250 ± 1,1401,560 ± 1,150<D.L.480 ± 21011 ± 11 (↓)<D.L.990 ± 279<D.L. (↓)<D.L.
  • a CN, Data from group given PBS and then infected with 107 C. neoformans.

  • b CN/IgG2a, Data from group given 1 mg of IgG2a and then infected with 107 C. neoformans.

  • c Sham, Mice given only PBS and not infected with C. neoformans.

  • d Arrows denote statistically significant (p < 0.50) increases (↑) or decreases (↓) in cytokine level relative to CN group. Values in table are means and SE of three to five mice per group.

  • e D.L., Detection limit in ELISA.