Table I.

IFN-γ release by cytokine-stimulated CD8+ T cellsa

Cytokine6 h24 h
NoneN/DbN/D
IFN-α/dN/D2.4 ± 0.8
IL-12N/D5.7 ± 3.0
IL-15N/DN/D
IL-18N/DN/D
IFN-α/d + IL-12 + IL-15N/D14 ± 1.4
IFN-α/d + IL-12N/DN/D
IFN-α/d + IL-1820 ± 2.5114 ± 32
IL-12 + IL-186.9 ± 2.31366 ± 188
IL-15 + IL-182.1 ± 0.9N/D
  • a Purified CD8+ T cells were stimulated with various combinations of IFN-α/d (1000 U/ml), IL-12 (100 ng/ml), IL-15 (100 ng/ml), or IL-18 (100 ng/ml) for 6 or 24 h. IFN-γ was detected in the cell-free supernatants by a europium-based anti-IFN-γ immunoassay. Results are expressed as mean IFN-γ (U/ml) ± SD of duplicate determinations of duplicate cultures.

  • b N/D = not detectable.