Treatment of T cells with IL-2, IL-7, and IL-15 but not IL-4 and IL-21, leads to up-regulation of IL-2Rα chaina
Media | IL-2 | IL-4 | IL-7 | IL-15 | IL-21 | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Isotype | Anti-CD25 | Isotype | Anti-CD25 | Isotype | Anti-CD25 | Isotype | Anti-CD25 | Isotype | Anti-CD25 | Isotype | Anti-CD25 | |
Anti-CD3 | 3.44 | 96.75 | 5.35 | 736.86 | 3.5 | 94.12 | 5.31 | 622.07 | 4.69 | 488.4 | 4.16 | 100.12 |
Anti-CD3/PD-L1.Fc | 3.12 | 46.38 | 4.49 | 307.03 | 4.05 | 51.41 | 4.18 | 246.99 | 4.46 | 272 | 4.34 | 51.42 |
a CD4+ T cells were activated with anti-CD3 Ab-coated microspheres in the presence of optimal concentrations of the indicated cytokines. At 72 h, cells were harvested and stained for IL-2Rα (CD25) expression as described in Materials and Methods (n = 2). Mean fluorescence values are shown, bold numbers indicate increases 4-fold above media control.