Table I.

Histopathology in C57BL/6 mouse lung overexpressing muOSM

Inflammatory Parametersa
TreatmentbDayMononuclear cellsNeutrophiliaEosinophiliaFibrosis/hyperplasiaGranuloma formationCollagen deposition
AdmuOSM7+++++++++++++++
14+++++++++++++
21+++++++
Addl707+
14++
21++
  • a Lung tissues were fixed with 10% buffered formalin, paraffin-embedded, sectioned, and stained with H&E, Congo red, Masson’s trichrome, and Elastin von Giesen stain. Tissues were examined in a blinded manner for the presence and extent of mononuclear cell accumulation, neutrophilia, and granuloma formation (H&E); fibrosis/hyperplasia and collagen deposition (H&E, Masson’s trichrome, and Elastin von Giesen); and eosinophilia (Congo red).

  • b C57BL/6 mice (minimum three mice per treatment three separate experiments) received 5 × 107 PFU AdmuOSM or Addl70 via intranasal administration and were sacrificed 7, 14, or 21 days later.