Histopathology in C57BL/6 mouse lung overexpressing muOSM
| Inflammatory Parametersa | |||||||
|---|---|---|---|---|---|---|---|
| Treatmentb | Day | Mononuclear cells | Neutrophilia | Eosinophilia | Fibrosis/hyperplasia | Granuloma formation | Collagen deposition |
| AdmuOSM | 7 | +++ | ++ | +++ | +++ | ++ | ++ |
| 14 | +++ | + | ++ | +++ | ++ | ++ | |
| 21 | ++ | − | + | ++ | + | + | |
| Addl70 | 7 | + | − | − | − | − | − |
| 14 | ++ | − | − | − | − | − | |
| 21 | ++ | − | − | − | − | − | |
a Lung tissues were fixed with 10% buffered formalin, paraffin-embedded, sectioned, and stained with H&E, Congo red, Masson’s trichrome, and Elastin von Giesen stain. Tissues were examined in a blinded manner for the presence and extent of mononuclear cell accumulation, neutrophilia, and granuloma formation (H&E); fibrosis/hyperplasia and collagen deposition (H&E, Masson’s trichrome, and Elastin von Giesen); and eosinophilia (Congo red).
b C57BL/6 mice (minimum three mice per treatment three separate experiments) received 5 × 107 PFU AdmuOSM or Addl70 via intranasal administration and were sacrificed 7, 14, or 21 days later.