Table III.

Phosphor imaging of 125I for wild-type and mutant human IgG1 molecules in BALB/c micea

MutantLiver PSL/mm2bLung PSL/mm2Heart PSL/mm2Spleen PSL/mm2Small Intestine PSL/mm2Large Intestine PSL/mm2
Wild type117 ± 36121 ± 37123 ± 10114 ± 635 ± 751 ± 6
M252Y/S254T/T256E35 ± 329 ± 530 ± 627 ± 1NDND
H433K/N434F/Y436H13 ± 112 ± 112 ± 112 ± 1NDND
  • a Measurements were carried out, as described in Materials and Methods, at Molecular Histology. Error was estimated as the SD for two independent series of measurements.

  • b Photo-stimulated luminescence (PSL) density. ND, not determined due to low signal. Background slide reading was 10.7 PSL/mm2. Background plate reading was 9.1 PSL/mm2.