Table II.

Cytokine and STAT requirements for induction of NK cell IFN-γ expression following MCMV infectiona

StrainTreatmentSerum IFN-γ (pg/ml)% IFN-γ + NK CellsNo. IFN-γ + NK Cells (× 103)
129UninfectedBLDBLDBLD
129MCMV2249 ± 35317 ± 1200 ± 30
129 IFN-αβRUninfectedBLDBLDBLD
129 IFN-αβRMCMV1895 ± 17614 ± 3220 ± 50
C57BL/6UninfectedBLDBLDBLD
C57BL/6MCMV4086 ± 18824 ± 5220 ± 40
C57BL/6 IL-12 p35UninfectedBLDBLDBLD
C57BL/6 IL-12 p35MCMV251 ± 28**5 ± 1**50 ± 20**
C57BL/6UninfectedBLDBLDBLD
C57BL/6MCMV576 ± 33030 ± 2230 ± 10
C57BL/6 STAT1UninfectedBLDBLDBLD
C57BL/6 STAT1MCMV5661 ± 50152 ± 3270 ± 60
129B6 F2UninfectedBLDBLDBLD
129B6 F2MCMV2578 ± 79548 ± 6200 ± 30
129B6 STAT4UninfectedBLDBLDBLD
129B6 STAT4MCMVBLD**17 ± 3**60 ± 50b
  • a Mouse strains were infected with MCMV for 1.5 days or left uninfected, and serum IFN-γ measured by ELISA. Splenic leukocytes were obtained, and NK cell IFN-γ expression was assayed as indicated in Materials and Methods. Three to four mice per group were used in experiments. Numbers shown are means ± SEM. ELISA limits of detection varied from experiment to experiment but were between 10 and 20 pg/ml. Flow cytometric limit of detection for specific IFN-γ expression within NK cells was 2%. BLD, Below limit of deletion.

  • b , p < 0.05 compared to infected immunocompetent animals.

  • c ∗, p < 0.02 compared to infected immunocompetent animals.