Table I.

Cytokine and STAT requirements for induction of NK cell cytotoxicity following MCMV infectiona

StrainTreatment% Specific Lysis (± SEM)b
129Uninfected1 ± 0
129MCMV30 ± 5
129 IFN-αβRUninfected1 ± 1
129 IFN-αβRMCMV3 ± 0c
C57BL/6Uninfected3 ± 1
C57BL/6MCMV13 ± 5
C57BL/6 IL-12 p35Uninfected1 ± 1
C57BL/6 IL-12 p35MCMV17 ± 7
C57BL/6Uninfected7 ± 0
C57BL/6MCMV28 ± 8
C57BL/6 STAT1Uninfected1 ± 1
C57BL/6 STAT1MCMV8 ± 3c
129B6 F2Uninfected4 ± 1
129B6 F2MCMV15 ± 3
129B6 STAT4Uninfected4 ± 1
129B6 STAT4MCMV27 ± 3
  • a Mouse strains indicated above were infected with MCMV for 1.5 days or left uninfected; splenic leukocytes were obtained and NK cell-dependent cytotoxicities were assayed as indicated in Materials and Methods. Three to four mice per group were used in experiments.

  • b Specific lysis was determined as described in Materials and Methods. The 100:1 E:T cell ratios (± SEM) are shown above, although ratios of 33:1, 11:1, 3:1, and 1:1 also were performed in experiments.

  • c , p < 0.02 compared to infected immunocompetent mice.